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42249 HEK293T cell transient overexpression lysate (as WB positive control)
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OR-LC423437
OriGene
https://www.origene.com.cn/catalog/proteins/over-expression-lysates/lc423437/septin-2-sept2-nm_001008491-human-over-expression-lysate
￥900.00
20 ug
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中文名称 42249 HEK293T cell transient overexpression lysate (as WB positive control)
英文名称 42249 HEK293T cell transient overexpression lysate (as WB positive control)
观察到的分子量 41.3 kDa
序列 TrueORF Clone [RC224864]
制剂 HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
宿主 HEK293T
来源 Human

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