双链RNA (dsRNA) 形成于含有互补序列的核酸链中，是mRNA体外转录过程中不需要的副产物。dsRNA 的最小化和去除对于mRNA体外转录 (IVT) 过程至关重要。
对于dsRNA的检测，USP推荐使用Dot-Blot法和ELISA法。金斯瑞凭借先进的抗体平台，首创了双J2抗体夹心的检测dsRNA ELISA 试剂盒（货号: L01020），该试剂盒不仅检测时间短，还能精准识别不同长度、不同修饰的dsRNA，为用户提供高效、快速的检测工具。
Double-stranded RNA(dsRNA) ELISA Kit (J2 based) is a Sandwich ELISA assay with two kinds of the monoclonal antibodies (J2) to sensitively and selectively detect dsRNA structure (larger than 30-40 bp) in mRNA molecules, independent of their nucleotide composition and sequence.
产品简介
| Linearity Range |
0.123 to 30 ng/mL |
| Sensitivity |
LLoQ: 0.123 ng/mL
ULoQ: 30 ng/mL |
| Precision |
Intra-assay: CV ≤ 10%
Inter-assay: CV ≤ 15% |
| Standard |
10 µg/mL of dsRNA with m1Ψ modification |
| Assay Time |
2 h 45 min |
| Storage |
The unopened kit is stable for at least 12 months from the date of manufacture at 2-8°C, and the opened kit is stable for up to 30 days from the date of opening at 2-8°C. |
| Kit Contents |
| Components |
Quantity |
Part.No |
| Capture Plate |
1 plate |
T1-80 |
| Standard Stock |
1 vial (25 μL) |
T1-10 |
| 100×Detection Antibody |
2 vials (Lyophilized) |
T1-20 |
| 10×HRP-Conjugated Antibody |
1 vial (1.50 mL) |
T1-30 |
| Sample Dilution Buffer |
1 bottle (50 mL) |
T1-60 |
| Detection Antibody Dilution Buffer |
1 bottle (12 mL) |
T1-61 |
| HRP-Conjugated Antibody Dilution Buffer |
1 bottle (12 mL) |
T1-62 |
| 20×Wash Solution |
1 bottle (60 mL) |
B-A1-71 |
| TMB Solution |
1 bottle (12 mL) |
B-A1-40 |
| Stop Solution |
1 bottle (6 mL) |
B-A1-50 |
| Plate Sealer |
2 pieces |
N/A |
|
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