单纯疱疹病毒蛋白16 (herpes simplex virus protein, VP16)是一种转录激活因子，能够激活下游基因表达。将VP16转录激活区与GAL4 BD融合表达，构建pGBKT7-VP16 (BD-VP16)载体，将其转化Y2HGold。VP16转录激活作用就会激活酵母体内报告基因HIS3、ADE2的表达，进而能够在组氨酸、腺嘌呤缺陷型培养基上正常生长。转录因子X分别与BD、BD-VP16融合表达，若转录因子X对下游基因为转录激活作用，会激活下游基因的表达。与含有BD的对照菌株相比，含有BD -X菌株能在缺陷型培养基上生长。若转录因子X对下游基因的转录抑制作用，进而会部分抑制VP16对下游基因的激活。与含有BD-VP16的对照菌株相比，含有BD-VP16-X菌株会抑制对下游的报告基因的激活，进而在缺陷型培养基上的生长会受到抑制。 Yujuan Chen et al. A MYB61-SWB9-KOs module regulates grain chalkiness via gibberellin biosynthesis in rice endosperm. PLANT BIOTECHNOLOGY JOURNAL. 2025. IF=10.1 Feng Tian et al. The sole active enzyme-encoding gene MlTPS1 in the trehalose biosynthesis pathway plays a crucial role in reproductive regulation of Magnolia liliiflora Desr.. INDUSTRIAL CROPS AND PRODUCTS. 2025. IF=5.6 Lanting Qi et al. The AmMYB35-AmFLS module mediates the accumulation of flavonol induced by drought stress in Astragalus membranaceus. Food Bioscience. 2025. IF=4.8