AB2020-50μl BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody 50μl 498.00元 AB2020-200μl BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody 200μl 1598.00元 来源 用途 交叉反应性 理论分子量 实际分子量 Rabbit WB, IF, IHC, ICC H, M, Rb 65kDa 65kDa WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry. H, Human; M, Mouse; Rb, Rabbit. BeyoAb™系列抗体为经深入测试的优质抗体，并配套提供Western一抗稀释液，可用于Western或其它适当用途的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整，NT, not tested)。 WB IP IF IHC ICC FC ChIP 1:1000-1:5000 NT 1:50-1:200 1:50 1:50-1:200 NT NT 本抗体相关应用的代表性检测效果图片请参见Fig. 1。 Fig. 1. BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody (AB2020) was used for Western blot, immunofluorescence and immunohistochemistry. A. Western analysis of lysates from indicated cell lines using BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody (AB2020) at 1:1000 dilution and BeyoWB™ HRP-labeled Goat Anti-Rabbit IgG (H+L) (P0948) at 1:500 dilution.20µg protein per lane was loaded and analyzed by BeyoWB™ 80min Electrophoresis, Transfer and Western Blot Kit (P0002). Exposure time, 2s. B. Immunofluorescence analysis of HeLa cells using BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody (AB2020) at a dilution of 1:50 and Cy3-labeled Goat Anti-Rabbit IgG (H+L) (A0516) at 1:250 dilution. a, c. Immunofluorescence image for NF-κB p65. b, d. Merged image of immunofluorescence for NF-κB p65 and nuclear staining using Antifade Mounting Medium with DAPI (P0131). C. Immunohistochemistry of a section from paraffin-embedded mouse spleen tissue using BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody (AB2020) at a dilution of 1:50 (bottom panel) compared to a negative control without primary antibody (Top panel). HRP-labeled Goat Anti-Rabbit IgG (H+L) (A0208) was used as secondary antibody at 1:1000 dilution. 抗体详细信息如下: About this Antibody Name BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody Category Polyclonal antibody (pAb); Primary antibody Isotype IgG Purification method Protein A affinity purification Positive samples HeLa; 293T Cellular location - Customer validation - About the Immunogen Immunogen Recombinant protein Sequence - Gene ID 5970 SwissProt Q04206 Synonyms NFKB3; Nuclear factor NF-kappa-B p65 subunit; RELA; TF65; Transcription factor p65; p65 Category Immunology and Inflammation Background NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis [1]. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one [2]. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression [3]. 包装清单： 包装 AB2020-50μl BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody 50μl AZ050 Western一抗稀释液 50ml — 说明书 1份 包装 AB2020-200μl BeyoAb™ NF-κB p65 Rabbit Polyclonal Antibody 200μl AZ200 Western一抗稀释液 200ml — 说明书 1份 保存条件： -20ºC保存，至少一年有效。Western一抗稀释液也可以4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项： 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4ºC保存。 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 为了您的安全和健康，请穿实验服并戴一次性手套操作。